Ginsenoside Rg1 ameliorates renal ischemia-reperfusion injury by inhibiting FABP1-regulated Nrf2/HO-1 pathway.
Chang Xiaodong, Zhang Lu, Zheng Guangyi, Zhang Hongyu, Xue Hen, Feng Wanyan
Abstract
Ginsenoside Rg1 (G-Rg1) can effectively ameliorate lipopolysaccharide-induced renal injury. The impact and mechanism of G-Rg1 in renal ischemia-reperfusion (I/R) injury are not yet understood. This study aimed to examine the role and mechanism of G-Rg1 in kidney I/R injury. The renal I/R injury mice and mouse kidney cells were applied as renal I/R injury models. Researchers analyzed the functions and mechanisms of G-Rg1 using techniques like cell proliferation, apoptosis, clone formation assay, ELISA, HE staining, immunohistochemical staining, Immunofluorescence staining, qRT-PCR, and Western blot analysis. Our findings indicate that G-Rg1 pretreatment protects against renal I/R injury by lowering serum creatinine and urea nitrogen levels, mitigating histological damage and apoptosis, and reducing inflammation and oxidative stress. These beneficial effects were accompanied by the suppression of fatty acid binding protein 1 (FABP1) and heme oxygenase-1 (HO-1) expression and the promotion of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation. However, the therapeutic effect of G-Rg1 was inhibited by FABP1 overexpression. The mechanism by which G-Rg1 ameliorates renal I/R injury may be related to the inhibition of FABP1 expression and thus regulation of the Nrf2/HO-1 pathway.
Key Findings
- Ginsenoside Rg1 pretreatment protects against renal ischemia-reperfusion injury by lowering serum creatinine and urea nitrogen levels.
- G-Rg1 mitigates histological damage, apoptosis, inflammation, and oxidative stress in renal I/R injury models.
- The protective effects involve suppression of FABP1 and HO-1 expression and promotion of Nrf2 nuclear translocation, with FABP1 overexpression inhibiting G-Rg1's therapeutic effect.
Clinical Significance
Ginsenoside Rg1 shows potential as a therapeutic agent to reduce kidney damage caused by ischemia-reperfusion injury through modulation of the FABP1-regulated Nrf2/HO-1 oxidative stress pathway.
Citation
Chang Xiaodong, Zhang Lu, Zheng Guangyiet al.. Ginsenoside Rg1 ameliorates renal ischemia-reperfusion injury by inhibiting FABP1-regulated Nrf2/HO-1 pathway. Renal failure. 2026-Dec.