Disruption of p62 ubiquitination by α-methylene-γ-butyrolactone enhances Keap1 lysosomal degradation for ulcerative colitis therapy.

Abstract

Ulcerative colitis (UC) is an intestinal inflammatory disorder primarily driven by M1 macrophage activation and oxidative stress. p62/Sequestosome-1, a multifunctional autophagy adaptor protein, plays a critical role in regulating autophagy and inflammation. α-Methylene-γ-butyrolactone (αMγB), a conserved structural motif found in anti-inflammatory sesquiterpene lactones from traditional medicinal herbs, was shown to suppress M1 macrophage activation and inflammation. This study aimed to investigate the role of p62 in UC pathogenesis and evaluate the therapeutic potential of αMγB. We found that genetic deletion of p62 aggravated disease severity in both acute pneumonia and UC mouse models. Treatment with αMγB alleviated UC symptoms by reprogramming macrophage polarization, reshaping the mucosal immune microenvironment, and suppressing inflammatory crosstalk between macrophages and epithelial cells. Mechanistically, αMγB covalently modified p62 at C291, preventing its ubiquitination and stabilizing it, which enhanced Keap1 binding and drove its autolysosomal degradation. This process released Nrf2, facilitated its nuclear translocation, and activated the expression of downstream antioxidant and anti-inflammatory genes. Importantly, αMγB established a positive feedback loop between Nrf2 and p62 that sustained p62 upregulation and further promoted Keap1 autolysosomal degradation. These protective effects were abolished in p62 (C291S) mutant mice or upon Nrf2 inhibition. Collectively, our findings demonstrated that αMγB ameliorated UC by specifically targeting p62 at C291, promoting its stabilization, and enhancing Keap1 degradation, thereby modulating macrophage polarization. These findings highlighted the therapeutic potential of αMγB and suggested that augmenting p62 function might represent a promising strategy for UC treatment.

Key Findings

• Genetic deletion of p62 worsened disease severity in ulcerative colitis (UC) and acute pneumonia mouse models.
• α-Methylene-γ-butyrolactone (αMγB) covalently modifies p62 at C291, preventing its ubiquitination and stabilizing it.
• Stabilized p62 enhances Keap1 binding and autolysosomal degradation, releasing Nrf2 to activate antioxidant and anti-inflammatory gene expression.
• αMγB treatment reprograms macrophage polarization and reshapes the mucosal immune microenvironment to alleviate UC symptoms.
• The protective effects of αMγB depend on p62 C291 and Nrf2 activity, establishing a positive feedback loop sustaining p62 upregulation.

Clinical Significance

Citation

Linghu Ke-Gang, Wang Dasong, Zhang Tianet al.. Disruption of p62 ubiquitination by α-methylene-γ-butyrolactone enhances Keap1 lysosomal degradation for ulcerative colitis therapy. Redox biology. 2026-Nov.